Sequential processing of lysosomal acid phosphatase by a cytoplasmic thiol proteinase and a lysosomal aspartyl proteinase.
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چکیده
منابع مشابه
Cathepsin B, the lysosomal thiol proteinase of calf liver.
Cathepsin B from calf liver was obtained by a method involving preparation of a lysosomal-mitochondrial pellet and treatment of this pellet with acetone. The material was extracted with an acid buffer, pH4.0, and then precipitated from the extract with acetone. The precipitate was dissolved in phosphate buffer, pH7.4, and subjected to gel filtration on Sephadex G-200 and G-100. The cathepsin B ...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1989
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1989.tb08480.x